GK104 ScienCell 人類細(xì)胞骨架重塑qPCR檢測試劑盒，Human Cytoskeletal Remodeling qPCR Array Kit

ScienCell 研究實驗室（ScienCell）是一家生物技術(shù)公司，其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

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GK104 ScienCell人類細(xì)胞骨架重塑qPCR檢測試劑盒，Human Cytoskeletal Remodeling qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌： ScienCell

產(chǎn)品貨號：GK104

產(chǎn)品名稱：GK104 ScienCell人類細(xì)胞骨架重塑qPCR檢測試劑盒，Human Cytoskeletal Remodeling qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK104 ScienCell人類細(xì)胞骨架重塑qPCR檢測試劑盒，Human Cytoskeletal Remodeling qPCR Array Kit， 產(chǎn)品說明：

ScienCell的GeneQuery™人類細(xì)胞骨架重塑qPCR陣列試劑盒（GQH-CYT）分析了參與細(xì)胞骨架重塑的88個關(guān)鍵基因，特別關(guān)注Rho GTP酶肌動蛋白細(xì)胞骨架重塑。 細(xì)胞骨架負(fù)責(zé)維持細(xì)胞形狀、支架和調(diào)節(jié)細(xì)胞內(nèi)運輸。 它還參與許多信號通路、細(xì)胞分裂、囊泡運輸和運動。 微絲主要是肌動蛋白聚合物，是細(xì)胞骨架的一部分，由GTP酶Rho家族調(diào)節(jié)。 如何對所包含的基因進行分類的簡要示例如下所示：

細(xì)胞骨架成分：ACTA1、ACTB、ACTG1、ACTN1、MYH10、MYL1

整合素： ITGA1， ITGA3， ITGAV， ITGB1， ITGB3

細(xì)胞外基質(zhì)成分：COL1A1，COL2A1，COL4A1，LAMA1，LAMB1

Rho信號分子：CAV1，CDC42，RAC1，RHOA，ROCK1，ROCK2

轉(zhuǎn)錄因子：CDX1、CDX2、SP1、TCF7、TCF7L1

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可識別并有效擴增特定靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的最佳退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Cytoskeletal Remodeling qPCR Array Kit (GQH-CYT) profiles 88 key genes involved in cytoskeletal remodeling with a particular focus on Rho GTPase actin cytoskeleton remodeling.  The cytoskeleton is responsible for maintaining cell shape, scaffolding, and regulating intracellular transport.  It is also involved in numerous signaling pathways, cellular division, vesicular trafficking, and motility.  Microfilaments, which are primarily actin polymers, are part of the cytoskeleton and regulated by the Rho family of GTPases.  Brief examples of how included genes may be categorized are shown below:

·         Cytoskeletal components: ACTA1, ACTB, ACTG1, ACTN1, MYH10, MYL1

·         Integrins: ITGA1, ITGA3, ITGAV, ITGB1, ITGB3

·         Extracellular matrix components: COL1A1, COL2A1, COL4A1, LAMA1, LAMB1

·         Rho signaling molecules: CAV1, CDC42, RAC1, RHOA, ROCK1, ROCK2

·         Transcription factors: CDX1, CDX2, SP1, TCF7, TCF7L1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類細(xì)胞骨架重塑 qPCR 陣列板布局*（8 個粗體和斜體對照）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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