GK028 ScienCell 人類Wnt信號(hào)靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室（ScienCell）是一家生物技術(shù)公司，其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實(shí)現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

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GK028 ScienCell人類Wnt信號(hào)靶基因qPCR檢測試劑盒，Human Wnt Signaling Target Genes qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號(hào)：GK028

產(chǎn)品名稱：GK028 ScienCell人類Wnt信號(hào)靶基因qPCR檢測試劑盒，Human Wnt Signaling Target Genes qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK028 ScienCell人類Wnt信號(hào)靶基因qPCR檢測試劑盒，Human Wnt Signaling Target Genes qPCR Array Kit， 產(chǎn)品說明：

ScienCell 的 GeneQuery™ 人類 Wnt 信號(hào)靶基因 qPCR 陣列試劑盒 （GQH-WTG） 旨在促進(jìn)對(duì) Wnt 信號(hào)激活有反應(yīng)的 88 個(gè)關(guān)鍵基因和 Wnt 信號(hào)誘導(dǎo)轉(zhuǎn)錄下游基因的基因表達(dá)譜分析。 該試劑盒包括由規(guī)范和非規(guī)范Wnt信號(hào)通路調(diào)節(jié)的基因。 如何根據(jù)其各自的功能對(duì)所包含的下游基因進(jìn)行分組的簡要示例如下所示：

轉(zhuǎn)錄因子：HNF1A、LEF1、TCF7L1、TCF7L2、FOSL1

細(xì)胞循環(huán)/生長：CCND1，CDKN2A，BIRC5，APC，TERC

動(dòng)力： S100A4， S100A6， NRCAM， KRT1， FN1

血管生成：EDN1，PLAU，VEGFA，NOTCH1，EDNRA

癌癥失調(diào)：TWIST1，RET，JUN，NEDD9，MLLT10

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個(gè)孔包含一個(gè)引物組，可識(shí)別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計(jì)的引物確保：（i）qPCR分析中的最佳退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery™ Human Wnt Signaling Target Genes qPCR Array Kit (GQH-WTG) is designed to facilitate gene expression profiling of 88 key genes responsive to Wnt signaling activation and genes downstream of Wnt signaling induced transcription.  This kit includes genes regulated by both canonical and noncanonical Wnt signaling pathways.  Brief examples of how included downstream genes may be grouped according to their respective functions are shown below:

·         Transcription factors: HNF1A, LEF1, TCF7L1, TCF7L2, FOSL1

·         Cell cycling/growth: CCND1, CDKN2A, BIRC5, APC, TERC

·         Motility: S100A4, S100A6, NRCAM, KRT1, FN1

·         Angiogenesis: EDN1, PLAU, VEGFA, NOTCH1, EDNRA

·         Cancer misregulation: TWIST1, RET, JUN, NEDD9, MLLT10

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類 Wnt 信號(hào)靶基因 qPCR 陣列板布局*（8 個(gè)粗體和斜體對(duì)照）

提示：基因選擇可能會(huì)根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購信息：

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